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Recombinant Mouse Thrombopoietin (CHO-expressed)

英文名：
Cas号：
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别 名
Cas号
M D L
分子式
分子量
产品参数
性状 Recombinant Mouse Thrombopoietin (CHO-expressed) 是一种造血因子，能够控制血小板生长。
Synonyms
rMuTPO; TPO ; 重组小鼠促血小板生成素 (CHO 细胞表达)
Species
Mouse

Source
CHO

Accession
P40226

Gene ID
21832

Molecular Weight
30-80 kDa

AA Sequence
SPVAPACDPR LLNKLLRDSH LLHSRLSQCP DVDPLSIPVL LPAVDFSLGE WKTQTEQSKA QDILGAVSLL LEGVMAARGQ LEPSCLSSLL GQLSGQVRLL LGALQGLLGT QLPLQGRTTA HKDPNALFLS LQQLLRGKVR FLLLVEGPTL CVRRTLPTTA VPSSTSQLLT LNKFPNRTSG LLETNFSVTA RTAGPGLLSR LQGFRVKITP GQLNQTSRSP VQISGYLNRT HG

Biological Activity
The ED50 is <2 ng /mL as measured by MO7e cells.

Appearance
Lyophilized powder.

Formulation
Lyophilized after extensive dialysis against PBS.

Endotoxin Level
<0.2 EU/μg, determined by LAL method.

Reconstitution
Reconstitute the lyophilized recombinant Murine Thrombopoietin (CHO-expressed) (rMuTPO) to 100 µg/mL using ddH2O or diluted with PBS.

Storage & Stability
Lyophilized recombinant Murine Thrombopoietin (CHO-expressed) (rMuTPO) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping
Room temperature in continental US; may vary elsewhere.

Background
Recombinant thrombopoietin reduces radiation- and chemotherapy-induced thrombocytopenia, enhances platelet recovery after bone marrow transplantation and increases the number of megakaryocyte precursor cells in stem cell harvests. Injection of thrombopoietin into animals stimulates the number, size and ploidy of bone marrow megakaryocytes and increases the platelet count up to ten-fold[1]. Superphysiological amounts of Thrombopoietin/TPO (>100 ng/mL) are able to directly activate platelet aggregation in vitro. Thrombopoietin/TPO also has significant effects on platelet adhesion under flow. Low Thrombopoietin/TPO concentrations (001-1 ng/mL) accelerate firm platelet adhesion to von Willebrand factor and prevent de-attachment at higher flow rates[2].
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