Recombinant Human Sonic Hedgehog, C24II

英文名:
Cas号:
检测信息查询
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别 名
Cas号
M D L
分子式
分子量
产品参数
性状 Recombinant Human Sonic Hedgehog, C24II 是一种重组人 sonic hedgehog 的变体。Recombinant Human Sonic Hedgehog 是一种分泌信号转导器,不仅负责早期肢体和神经发育过程中前后轴的图形化,还负责在后期发育阶段产生细胞类型的多样性。
Synonyms
rHuSonic Hedgehog, C24II; HHG1 ; 重组人 Sonic Hedgehog, C24II
Species
Human

Source
E. coli

Accession
Q15465

Gene ID
6469

Molecular Weight
Approximately 19.7 kDa

AA Sequence
IIGPGRGFGK RRHPKKLTPL AYKQFIPNVA EKTLGASGRY EGKISRNSER FKELTPNYNP DIIFKDEENT GADRLMTQRC KDKLNALAIS VMNQWPGVKL RVTEGWDEDG HHSEESLHYE GRAVDITTSD RDRSKYGMLA RLAVEAGFDW VYYESKAHIH CSVKAENSVA AKSGG

Biological Activity
The ED50 is <2 μg/mL as measured by C3H/10T1/2 (CCL-226) cells, corresponding to a specific activity of >500 units/mg.

Appearance
Lyophilized powder.

Formulation
Lyophilized after extensive dialysis against PBS.

Endotoxin Level
<0.2 EU/μg, determined by LAL method.

Reconstitution
Reconstitute the lyophilized recombinant Human Sonic Hedgehog, C24II (rHuSonic Hedgehog, C24II) to 100 µg/mL using ddH2O.

Storage & Stability
Lyophilized recombinant Human Sonic Hedgehog, C24II (rHuSonic Hedgehog, C24II) is stored at -20°C. After reconstitution, it is stable at 4°C for 2 weeks or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping
Room temperature in continental US; may vary elsewhere.

Background
Sonic Hedgehog (SHH) is a secreted factor. The protein is heavily modified through cleavage and posttranslational modification and its release from producing cells can also be modulated by interactions with Dispatched, Scube2, and heparan sulfate proteoglycan[1][2].
贮存
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