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Recombinant Mouse Tumor Necrosis Factor alpha, His
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| 性状 | Recombinant Mouse Tumor Necrosis Factor alpha, his 是带有标签的重组小鼠肿瘤坏死因子α (TNF-α)。Recombinant Mouse Tumor Necrosis Factor alpha 是炎症的主要介质，参与了许多传染病和寄生虫病。
Synonyms rMuTNF-α, His; Cachectin; TNFSF2 ; 重组小鼠肿瘤坏死因子α，His 标签 Species Mouse Source E. coli Accession P06804 Gene ID 21926 Molecular Weight Approximately 18.3 kDa AA Sequence MHHHHHHMLR SSSQNSSDKP VAHVVANHQV EEQLEWLSQR ANALLANGMD LKDNQLVVPA DGLYLVYSQV LFKGQGCPDY VLLTHTVSRF AISYQEKVNL LSAVKSPCPK DTPEGAELKP WYEPIYLGGV FQLEKGDQLS AEVNLPKYLD FAESGQVYFG VIAL Biological Activity The ED50 is <0.05 ng/mL as measured by L-929 mouse fibrosarcoma cells, corresponding to a specific activity of >2 × 107 units/mg. Appearance Lyophilized powder. Formulation Lyophilized after extensive dialysis against PBS. Endotoxin Level <1.0 EU/μg, determined by LAL method. Reconstitution Reconstitute the lyophilized recombinant Mouse Tumor Necrosis Factor alpha, His (rMuTNF-α, His) to 100 µg/mL using ddH2O. Storage & Stability Lyophilized recombinant Mouse Tumor Necrosis Factor alpha, His (rMuTNF-α, His) is stored at -20°C. After reconstitution, it is stable at 4°C for 4 weeks or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping Room temperature in continental US; may vary elsewhere. Background Tumor necrosis factor-α (TNF-α) is a pleiotropic cytokine produced by a wide variety of cell types of mostly hematopoietic, but also of nonhematopoietic, origin. TNFα is instrumental in the immune elimination of various infectious agents such as Candida albicans, Listeria monocytogenes, or mycobacteria and exerts potent proinflammatory effects, e.g. by inducing the expression of adhesion molecules such as VCAM-1, intercellular adhesion molecule 1 (ICAM-1), or E-selectin on endothelial cells and other cell types[1][2]. |
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